Journal: Bio-protocol
Article Title: Computational Cellular Mathematical Model Aids Understanding the cGAS-STING in NSCLC Pathogenicity
doi: 10.21769/BioProtoc.5223
Figure Lengend Snippet: A. Launch MATLAB R2010b. B. In the Command window, type “SIMBIOLOGY” or “symbiology” to launch the SimBiology toolbox. C. Click on Diagram view to visualize the canvas. D–E. From the Block Library Browser , drag and drop the compartment and label it accordingly. F. Drag and drop species and reaction in the compartment. G. Press CTRL and connect species 1 (PI3K for representation) with the reaction. Repeat the step with species 2 (AKT). Double-click on the reaction and set the parameters, which mainly include kinetic law, kinetic law parameter, value, units, and substrate concentration. Make sure that the Active reactive check box is ticked. H. In Tasks , select Add model task to the untitled and select Simulate model . I. In the Configuration settings and data logging option, select and tick the species in the checkbox of log column. J. In the Settings option adjacent to data logging , select the solver type as ode15s (stiff/NDF) and change the stop time to 100.0 simulation time (seconds). K. Press CTRL+T and observe the concentration vs. time plot for the reaction.
Article Snippet: MATLAB v7.11.1.866's SimBiology toolbox was used for the reconstruction of the mathematical model.
Techniques: Blocking Assay, Concentration Assay